Evaluasi ketahanan galur melon madura (Cucumis melo L.) terhadap cucumber mosaic virus

نویسندگان

چکیده

Tanaman melon (Cucumis melo L.) merupakan salah satu tanaman buah-buahan yang penting dan ini banyak ditanam di berbagai negara. Permintaan produksi buah meningkat harus seimbang dengan ketersediaan benih melon. Dalam budidaya terdapat beberapa kendala satunya pada waktu dibutuhkan. Budidaya tidak terlepas dari hambatan pertumbuhan karena gangguan hama penyakit. Mengatasi permasalahan diperlukan perakitan varietas unggul tahan. Varietas diperoleh tetua Sehingga penelitian bertujuan untuk mengevaluasi galur-galur lokal Madura memiliki ketahanan terhadap Cucumber mosaic virus (CMV). Penelitian dilakukan greenhouse penanaman melon, Program Studi Agroekoteknologi Fakultas Pertanian Universitas Trunojoyo Madura. Sampel daun bergejala penyakit dibawa ke laboratorium ekstrak genom. Amplifikasi DNA RT-PCR menggunakan sepasang primer CMV-1F CMV-1R. Hasil konfirmasi menunjukkan sampel positif terinfeksi CMV. Berdasarkan hasil percobaan secara umum galur G16 tingkat terbaik dibandingkan lain pembanding. Selain itu, G8 juga cukup tahan berpotensi digunakan sebagai

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ژورنال

عنوان ژورنال: Agrovigor

سال: 2022

ISSN: ['1979-5777', '2477-0353']

DOI: https://doi.org/10.21107/agrovigor.v15i2.14886